rIdeZ A0211

Description: rideZ, MS grade, is an engineered recombinant protease overexpressed in Escherichia coli. It cleaves 

specifically at a single recognition site below the hinge region to produce homogenous F(ab’)2 and Fc fragments. Both 

IdeS and IdeZ proteases effectively cleave human IgG1, IgG2, IgG3, and IgG4, monkey, sheep, rabbit, humanized and 

chimeric IgG, and Fc fusion proteins. However, only the IdeZ protease cleaves mouse IgG2a and IgG3.

Physical Appearance: Lyophilized powder

Molecular Weight: 37 kDa

Resuspension Buffer (reference): 40 μL double-distilled water from user

Digestion Buffer: 10X 50 mM PBS, 150 mM NaCl (pH 6.6) solution

Storage Conditions: Powder at -20 °C

Shelf Life: 12 months at -20 °C

pH Range: Maximally active at pH 6-8

Application: rIdeS protease is an IgG-specifific degradation enzyme to produce homogeneous F(ab’)2  and Fc fragments. 

In-Solution Protein Digestion Protocol (Recommended):

1. Mix 5 μL 10X enzyme digestion buffer and double-distilled water.

2. Add 10 μL 10 μg/μL mAb samples to the mixture.

3. Add 4 μL 0.5 μg/μL rIdeS to the mixture to total reaction volume of 50 μL.

4. Incubate at 37 °C for 30 min.

Purity: > 99.5%

Specificity: > 99.3% purity of mAb antibody samples after rIdeS digestion and elution with mAB pH ion mobility buffer, 

analyzed by UPLC chromatography with SCX 2.1*50 mm column.

Activity: 50 units/μg

Unit Definition: One unit is the amount of 1 μg rIdeZ required to produce 50 μg substrate IgG antibody at 37 °C in 30 min.

MALDI-TOF Analysis: Purified rIdeZ was analyzed by HCCA matrix laser analytical mass spectrometry. No impurity peak 

was found.